| Step | Temperature ˚C | Time | Number of cycles |
| Initial denaturation | 94 | 5 minutes | 1 |
| Denaturation | 94 | 30sec | 40 cycles |
| Annealing | 56 | 1mins | 1 |
| Extension | 72 | 1mins | 1 |
| Final extension | 72 | 5mins | 1 |
| Final hold | 4 |